Soil bacteria made to produce valuable fuel from waste
Washington, August 21 (ANI): Scientists at MIT have genetically modified a humble soil bacterium to persuade it to make fuel - specifically, a kind of alcohol called isobutanol that can be directly substituted for, or blended with, gasoline.
Unlike some proposed biofuels, isobutanol can be used in current engines with little or no modification, and has already been used in some racing cars.
The microbe called Ralstonia eutropha has a natural tendency, whenever it is stressed, to stop growing and put all its energy into making complex carbon compounds.
Christopher Brigham, a research scientist in MIT's biology department who has been working to develop this bioengineered bacterium, is currently trying to get the organism to use a stream of carbon dioxide as its source of carbon, so that it could be used to make fuel out of emissions.
Brigham explains that in its natural state, when the microbe's source of essential nutrients (such as nitrate or phosphate) is restricted, "it will go into carbon-storage mode," essentially storing away food for later use when it senses that resources are limited.
"What it does is take whatever carbon is available, and stores it in the form of a polymer, which is similar in its properties to a lot of petroleum-based plastics," Brigham said.
By knocking out a few genes, inserting a gene from another organism, and tinkering with the expression of other genes, Brigham and his colleagues were able to redirect the microbe to make fuel instead of plastic.
While the team is focusing on getting the microbe to use CO2 as a carbon source, with slightly different modifications the same microbe could also potentially turn almost any source of carbon, including agricultural waste or municipal waste, into useful fuel. In the laboratory, the microbes have been using fructose, a sugar, as their carbon source.
At this point, the MIT team - which includes chemistry graduate student Jingnan Lu, biology postdoc Claudia Gai, and is led by Anthony Sinskey, professor of biology - have demonstrated success in modifying the microbe's genes so that it converts carbon into isobutanol in an ongoing process.
Now, the researchers are focusing on finding ways to optimize the system to increase the rate of production and to design bioreactors to scale the process up to industrial levels.
The research was reported this month in the journal Applied Microbiology and Biotechnology. (ANI)
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